This study revealed that remimazolam promoted the cell-cycle progression and proliferation of HCT8 colon cancer cells by upregulating CDK1, PTTG1, CDC25C, CDK4, PLK1, PCNA, Ki67, RNASEH2B, FEN1, Cyclin D1,CD44 CDK2, CDKN3, CDC45, IQGAP3, and CDK6. Here, RNASEH2B is linked to malignant colon neoplasm.