Over the past years, the in situ hybridization-based technique RNAscope has been increasingly used in research and clinical studies due to its sensitivity and specificity, which have been evaluated in the context of pathologic diagnostics.21, –23 RNAscope has been used to evaluate TRPA1 and TRPV1 mRNA abundance in neuronal cells from the peripheral24, –26 and the central nervous system,27, –29 as well as non-neuronal tumor cells.30 However, whether there is a correlation between mRNA and functional responses has not been assessed before. This evidence concerns the gene TRPV1 and neoplasm.