Based on our results, we speculated that miR-486-3p may target TCF12 and mediate the malignant progression of glioma; therefore, an in vitro rescue experiment was conducted to evaluate the regulatory role of LINC00606 via its direct sponging of miR-486-3p and subsequent influence on TCF12. Firstly, to demonstrate the effect of LINC00606 on the regulation of the miR-486-3p target gene TCF12, we transfected U251 cells with the miR-486-3p mimic, and the mRNA and protein expression levels of TCF12 decreased significantly. The gene discussed is LINC00606; the disease is glioma.