Statistical analysis shows that in comparison with the Control, the expression levels of p-PI3K/PI3K, p-AKT/AKT, and p-GSK3β/GSK3β in U251 cells were significantly reduced following overexpression of ATP11B, and the expression level of PTEN (as a negative regulator of tumor suppressors and PI3K/AKT) was significantly increased. This evidence concerns the gene GSK3B and neoplasm.