To investigate the effect of ATP11B on the PI3K/AKT signaling pathway in glioma, U251 cells were transfected with OE ATP11B or siATP11B, and the protein expression levels of p-PI3K, PI3K, p-AKT, AKT, PTEN, p-GSK3β, and GSK3β were investigated by immunoblotting (Fig. 5p; Supplementary Fig. S9a–d). Here, GSK3B is linked to glioma.