Additionally, the CD4/CD8 ratio is one of biomarkers for immunological dysfunctions such as immunodeficiency and autoimmunity; among the in wild type, Foxn1Cas9, Rosa26_ms1, and Rosa26_ms2 mice splenocytes there were no significant differences the CD4/CD8 ratio (Fig. S8 and Fig. 5F). The gene discussed is CD8A; the disease is immunodeficiency disease.