We found that differential PC1 calling, reflecting GSC-specific differences in eu-/heterochromatin, broadly separated many (but not all) CL/DEV from MES/INJUR lines, yet this was not a reflection of the presence or absence of major GBM driver mutation like amplification of EGFR or loss of CDKN2A/B and PTEN (Supplementary Fig. 4c). This evidence concerns the gene CDKN2A and glioblastoma.