SEC31A and amyotrophic lateral sclerosis: Interestingly, we did not observe any significant changes in percentage area of total Sec31A puncta between control and patient using either statistical method (student’s t-test p = 0.2827; linear mixed effect model p = 0.3763), suggesting that despite the difference in puncta size and number, the area of Sec31A puncta were similar between the control and ALS patients (Fig. 7D).