To confirm that post-mortem fixation delay did not affect immunoreactivity and the localisation of Sec31A, we examined whether there were any significant differences in Sec 31 puncta count/area, % area and average size between the two patients with post-mortem intervals (PMIs) at the top and bottom of range in this study: ALS-C9orf72 1 and ALS-C9orf72 3 (PMI 7 h and 99 h, respectively). This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.