Since PNO1 knockout inhibited the expression of PTGS2, a gene involved in inflammation, we, therefore, sought to examine the effects of inhibiting PNO1 on the expression of inflammatory cytokines (IL‐1α and IL‐8) and chemokine (CXCL‐8) in HCC cells. The gene discussed is PTGS2; the disease is hepatocellular carcinoma.