The most abundant include hypoxia regulated biglycan,72 dihydropyrimidinase-related protein 5, which was connected with enhanced invasion and proliferation of PCa,73 and pentraxin-related protein PTX3, that was shown to have a role in acceleration of breast cancer metastasis when present in EVs.74 Therefore, it could be speculated that this hypoxia specific cargo in sEVs could contribute to the observed increased secretion of sEVs by the HTB-41 B2 cells. This evidence concerns the gene PTX3 and breast cancer.