RSPO1 and Barrett esophagus: First records of BE organoids appeared in 2011, when human metaplastic BE specimens were digested and seeded in Matrigel with a basal culture medium overlaid with gastrin, nicotinamide, A83-01, SB202190, Wnt-3A, noggin, EGF and R-spondin-1, leading to a successful passage over 1 month (5 passages) (Sato et al., 2011).