In addition, electroacupuncture preconditioning can prevent cerebral ischemia/reperfusion injury through cannabinoid CB1 receptor (CB1R)-mediated phosphorylation of glycogen synthase kinase-3β (GSK-3β) (Wei et al., 2014; Xu et al., 2015; Li et al., 2022). This evidence concerns the gene CNR1 and Cerebral ischemia.