Our outcomes of immunofluorescence staining revealed notable disparities in the protein expression levels of CHMP4C, p-GSK3β, and β-catenin among the U2OS, HOS, and MG63 osteosarcoma cells, with significantly higher expression observed in these cells compared to hFOB1.19 cells (Figures 6B−D). This evidence concerns the gene CHMP4C and osteosarcoma.