In contrast, murine pancreatic cancer cell line (3275) revealed highest mRNA expression of CerS1 in CBD treatment group (2.44-fold upregulation), followed by combination of gemcitabine and CBD (2.10-fold upregulation), triple combination group (1.83-fold upregulation) and gemcitabine monotherapy showed a 1.39-fold upregulation (Fig. 2a). The gene discussed is CERS1; the disease is familial pancreatic carcinoma.