Because many NSCLC-CTCs lack expression of epithelial markers such as EpCAM [4], we purposely chose an unbiased approach based on a negative selection of CTCs by targeting hematopoietic cells (HPCs) for CTC enrichment from DLAs prior to sc RNA sequencing (scRNAseq) (Fig. 1A). This evidence concerns the gene EPCAM and non-small cell lung carcinoma.