To further determined the in vivo effect of ACSL4 T679 phosphorylation, we constructed tumor-bearing mice by subcutaneously injecting ACSL4TetOn-ACSL4−/− (ACSL4 WTTet) HONE1 cells and ACSL4-T679ATetOn-ACSL4−/− (ACSL4 T679ATet) HONE1 cells, followed by gene expression induction with doxycycline (Dox). This evidence concerns the gene ACSL4 and neoplasm.