Many pathogenic gene mutations common to breast cancer [23], such as Pten, Brca2, Atm, Cdh1, Chek2, Nf1, Arid1a, Pik3ca, and Esr1, revealed no alterations between NT2.5 and NT2.5-LM (Fig. 2a). Here, PIK3CA is linked to breast carcinoma.