In the NT2.5 syngeneic model, NT2.5 cells are implanted in the mammary fat pad of adult female NeuN mice, after which the maximum allowable volume of 1.5 cm3 is reached in 4–5 weeks [13, 16, 22], prior to the establishment of metastatic disease and preventing efficient study of metastatic tumor microenvironments (TMEs). Here, RBFOX3 is linked to metastatic neoplasm.