To ascertain that the binding epitope of P2A5 on B7-H3 remains unaffected by the glycosylation in murine and human cells, we introduced either the human (4-Ig) or murine (2-Ig) B7-H3 molecules into a B7-H3–negative murine colon cancer cell line (CT26) and a B7-H3 knocked-out gastric adenocarcinoma cell line (MKN7). This evidence concerns the gene CD276 and malignant colon neoplasm.