Finally, as B7H3.CAR EBVSTs recovered from both HT-29 colorectal cancer and NCI-N87 gastric cancer cell line–derived xenografts displayed increased expression of PD-1 and/or TIM-3 (Supplementary Fig. S4B and S4D), which are also markers associated with T-cell exhaustion, we sought to evaluate long-term persistence and functionality of B7H3.CAR EBVSTs in vivo. The gene discussed is CD276; the disease is colorectal cancer.