- RSV reduced the levels of glial fibrillary acidic protein, TNF-α, IL-1β, and NF-Κβ.- RSV decreased COX-2 expression and suppressed ROS production- Totally, RSV reduced the spinal COX-2-mediated ROS generation and inflammatory response, suppressed astrocytic activation, and alleviated OXA-induced neuropathic pain. The gene discussed is TNF; the disease is neuropathic pain.