In addition, beside the therapy of cerebral ischemia, the application of injectable ECM hydrogels was wide including skin wound healing, myocardial infarction, bone and cartilage defect, etc. Currently, the verification of effectiveness of EBP-bFGF/ECM hydrogel was only selected MCAO animal model that was another limitation of this study. This evidence concerns the gene FGF2 and brain ischemia.