The average AUC of the RF model to distinguish HT from LH was 0.998 ± 0.002 (accuracy: 0.991 ± 0.007, sensitivity: 0.994 ± 0.010, specificity: 0.988 ± 0.014, precision: 0.988 ± 0.013, F1 score: 0.991 ± 0.007, Supplementary Data 2). The gene discussed is PLOD1; the disease is hematocrit.