In this study, the expression levels of GLUT1, LDHA, PFKFB3, PKM2 and HK2 were elevated in Cetuximab resistant cells, and downregulating circHIF1A reduced the promoter activity and expression of GLUT1 and LDHA, suggesting that the increased level of glycometabolism, especially glycolysis, in Cetuximab resistant CRC cells is closely related to the intracellular high level of circHIF1A. Here, PFKFB3 is linked to colorectal carcinoma.