NMT1 and chronic myelogenous leukemia, BCR-ABL1 positive: To investigate the role of NMT1, we bypassed the essential dependency of NMT1 on cells by creating a doxycycline-inducible NMT1 knockout system that regulates the expression of NMT1 gRNA in near-haploid chronic myeloid leukemia (CML) HAP1 cells constitutively expressing Cas9 [34].