These genes were GFRA1, SHTN1, PDZD8, PCDH19, BEX3, TUBB2B, CNTN1, NDRG1 and BASP1. Remaining six of the downregulated genes by C2A–2B–3–4 we validated by qPCR code for cancer-associated factors. The gene discussed is SHTN1; the disease is cancer.