Moreover, we further found that phosphorylation of P70S6K also could be detected after treated with the mTOR inhibitor rapamycin in the co-transfection of YANK2 and P70S6K (Fig. 6G), and the p-p70S6K T389 level in YANK2-positive glioma was also increased by IHC assay (Fig. 6H). The gene discussed is RPS6KB1; the disease is central nervous system cancer.