Moreover, immunofluorescence staining of NB tissues suggests that cells positive for HK3 co-express with cells positive for CD163 in the same region, and the co-expression level of HK3 and CD163 increased in INSS IV (Fig. 1j), indicating that the number of infiltrated CD163 macrophages have a positive correlation with HK3 level in NB. This evidence concerns the gene HK3 and neuroblastoma.