To further research on metabolic diseases, we still use HepG2 cells and treat them with TNF-α (10 ng/ml) for 24 h, and intracellular lipid accumulation increased significantly treated with TNF-α (Fig. 1A, B), Meanwhile, the expression of lipogenesis genes such as Acetyl-CoA carboxylase 1 (ACC1), FAS, Forkhead box protein 1 (FOXO1), as well as lncRNA MEG3 significantly increased (Fig. 1C). This evidence concerns the gene MEG3 and metabolic disease.