Given the critical importance of IFNγ in host defenses against cryptococcosis [25–27], in the final set of experiments we isolated lung cells, splenocytes, and peripheral blood mononuclear cells (PBMCs) from mice which were vaccinated and/or infected, and then measured IFNγ production following ex vivo stimulation with HK C. neoformans. The gene discussed is IFNG; the disease is cryptococcosis.