1. Three distinct leukemic cell populations identified: CD34+CD117dim blasts, CD34+CD117bri blasts, and abnormal myeloid cells with partial maturation (AM).2.CD34+CD117dim cells overexpress ell migration and adhesion genes, while CD34+CD117bri cells overexpress cell cycle and DNA replication genes.3. CD34+CD117dim cells show higher LSC17 score compared to CD34+CD117bri cells4. A high proportion of CD34+CD117dim cells in t(8;21) AML patients predicts inferior outcomes. The gene discussed is CD34; the disease is acute myeloid leukemia.