Doubling times of RFWD3Δ/Δ cells were significantly increased compared with controls (mean doubling time 16.1h control VS 20.8h RFWD3 Δ/Δ clone 3, p=0.028; mean doubling time 16.1h control VS 29.8h RFWD3 Δ/Δ clone 44, p<0.0001) (Figure 4D), demonstrating that deficiency of RFWD3 results in impairment of tumour cell proliferation. This evidence concerns the gene RFWD3 and neoplasm.