HLF and cancer: When we used the DNMIVD database to investigate the methylation pattern of the HLF gene promoter, we discovered that, as shown in Table S4, between 22 different kinds of cancers, the promoter region of the HLF gene was significantly hypermethylated in BLCA (Beta difference = 0.175 and adjusted p-value = 2.49e-04), COAD (Beta difference = 0.111 and adjusted p-value = 1.16e-04), and PRAD (Beta difference = 0.200 and adjusted p-value <0.0000).