Neighbourhood analysis revealed that CD8+ T cells in tumours treated with PI3K/mTORi or PI3K/mTORi+PD‐1i were in closer proximity to CD4+ T cells and expressed elevated levels of cytotoxicity markers when adjacent to CD4+ T cells, highlighting the supportive role of CD4+ T cells in maintaining the cytotoxic capabilities of CD8+ T cells (Figure 4E). This evidence concerns the gene PIK3CA and neoplasm.