Mechanistically, compound 20 binds to ALKBH5 by occupying the m6A-binding pocket, exerting anti-AML cell proliferation effects through modulation of the cell cycle, E2F targets, G2M checkpoint, and MYC targets (MOLM-13 IC50 = 0.49 μM, MV4-11 IC50 = 1.2 μM). This evidence concerns the gene ALKBH5 and acute myeloid leukemia.