For the first time, we quantified the stemness of BPH tissues using ssGSEA and found a strong positive correlation between the stemness of BPH tissues and the TGF-β pathway score, revealing that BPH tissues with high TGF-β scores may have a greater degree of stemness, as they can recruit multipotential MSCs to the prostate, providing direct validation of the relationship between BPH tissue stemness and TGF-β. This evidence concerns the gene TGFB1 and benign prostatic hyperplasia.