HSP90B1 and nasopharyngeal carcinoma: The efficacious transfection was validated, as evidenced in Fig. 2A, B. Assessments of cellular proliferation, utilizing colony formation and EdU incorporation assays, revealed a clear dichotomy: HSP90B1 knockdown significantly impeded, while its overexpression facilitated, the proliferative capacity of NPC cells (Fig. 2C, D).