We performed EMSA analysis using biotin labeled promoter sequence of BRCA1, BRCA2, and p16 gene retrieved from EMBOCpG plot for MBD1, MBD2, and MeCP2 proteins binding isolated from MCF-10A, MCF-7, T-47D & MDA-MB-231 human breasts normal and cancer cell line. This evidence concerns the gene MBD2 and cancer.