Shift of bands, as well as a supershift of MBD1, MBD2 & MeCP2, proteins and BRCA1 promoter along with protein specific primary Antibody complexes in an PAGE gel, confirmed the MBD proteins binding on BRCA1 promoter sequence in MCF-10A, MCF-7, T-47D & MDA-MB-231 breasts normal and cancer cell line but no binding was observed on BRCA2 and p16 promoter sequences as there was no bands observed on gel in the cell lines mentioned above (Fig. 2A-I). The gene discussed is BRCA1; the disease is cancer.