Next, for survival analysis, we re-classified non-mucinous adenocarcinoma cases of each grade group into HNF4α-positive and HNF4α-negative groups: HNF4α-positive grade 3 group (n = 6), HNF4α-negative grade 3 group (n = 50), HNF4α-positive grade 2 group (n = 9), HNF4α-negative grade 2 group (n = 119), and HNF4α-negative grade 1 group (n = 29), as well as the variant group (n = 17). This evidence concerns the gene HNF4A and colon mucinous adenocarcinoma.