ASS1 and breast carcinoma: In addition, the IHC results showed that ASS1 was negatively associated with breast cancer recurrence (Fig. 2G, H), whereas PHGDH was positively associated with breast cancer recurrence (Fig. 2G, I), and ASS1 was negatively associated with PHGDH in TNBC patient tissue (Fig. 2J, S5A).