Our results also showed that ASS1 knockout and knockdown promote TNBC cell proliferation in vitro and in vivo; however, ASS1 overexpression suppressed cell proliferation, which is consistent with a recent study showing that ASS1 knockdown promotes while ASS1 overexpression inhibits MDA-MB-231 cell growth and tumor development [13]. The gene discussed is ASS1; the disease is neoplasm.