To further verify the regulatory relationship between CEBPB and SERPINA1 and their effects on colorectal cancer proliferation, we transfected RKO and Caco2 cells with si-NC and Control, si-CEBPB and Control, and si-CEBPB and SERPINA1, respectively, and performed colony formation assays. The gene discussed is SERPINA1; the disease is colorectal cancer.