Of the 95 lymph node metastases, analyzable samples were available for 70 (TLR1), 72 (TLR2), 77 (TLR4), 58 (TLR5), 76 (TLR6), 72 (TLR7), 76 (TLR8) and 70 (TLR9) cases. This evidence concerns the gene TLR5 and metastatic malignant neoplasm in the lymph nodes.