Additionally, an asthma mouse model was established to quantify the levels of TGF-β1 in serum using enzyme-linked immunosorbent assay (ELISA), and evaluate the expression levels of TGF-β1, total-STAT3, p-STAT3, and CTGF proteins in lung tissue through Western blot analysis. The gene discussed is TGFB1; the disease is asthma.