The Western blot and IHC analysis results revealed that the expression of BCL2L11 in the liver tissue of HFD induced NAFLD mice was significantly downregulated, while the expression of NAGS, HDHD3, and RMND1 proteins was significantly upregulated (P < 0.01) (Fig. 10A and B). This evidence concerns the gene NAGS and metabolic dysfunction-associated steatotic liver disease.