TNF and metabolic dysfunction-associated steatotic liver disease: Further functional enrichment analysis revealed that DNA replication, base excision repair, peroxisome, ubiquinone and other terpenoid-quinone biosynthesis, and primary bile acid biosynthesis were significantly strengthened in NAFLD samples, while osteoclast differentiation, IL-17 signaling pathway, rheumatoid arthritis, and TNF signaling pathway were significantly strengthened in healthy liver samples (Fig. 1D and E).