The Western blot and IHC analysis results revealed that the expression of BCL2L11 in the liver tissue of HFD induced NAFLD mice was significantly downregulated, while the expression of NAGS, HDHD3, and RMND1 proteins was significantly upregulated (P < 0.01) (Fig. 10A and B). The gene discussed is BCL2L11; the disease is metabolic dysfunction-associated steatotic liver disease.