Our study demonstrated a significant reduction in both the mRNA and protein levels of TMBIM6 in ESCC cells upon SNHG1 knockdown, which was reversed by the miR-216a-3p inhibitor, thereby confirming the presence of the SNHG1/miR-216a-3p/TMBIM6 axis. The gene discussed is SNHG1; the disease is esophageal squamous cell carcinoma.