We assessed the expressions of PSAT1, E-cadherin, α-SMA, AKT, and p-AKT in SiPSAT1-transfected colorectal cancer cells treated with the PI3K/AKT signaling pathway inhibitor LY294002.Previous research has established that PSAT1 knockdown significantly elevates p-AKT expression in HCT116 and SW480 cells. The gene discussed is ACTA1; the disease is colorectal cancer.