In this study, the correlations between the expressions of IDO1 and genes encoding rate‐limiting glycolytic enzymes such as HK2, PK, LDHA, and GLUT family members in PC patients were analyzed with bioinformatic tools, and the regulatory effects of IDO1 on glycolysis as well as on glucose uptake were explored in mouse and human PC cells. Here, SLC2A1 is linked to pachyonychia congenita.