Importantly, in parallel with the GFAP marker, it would be interesting to investigate other neurotransmitter measures such as for glutamate staining (e.g. vesicular glutamate transporter; vGluT1), as astrocytes regulate glutamatergic homeostasis implicated in alcohol abuse (Adermark and Bowers 2016) as well as co-express vGluT1 in their processes (Verkhratsky et al. 2016). Here, GFAP is linked to alcohol abuse.