A transposon system containing plasmids encoding oncogenes along with transposase‐induced spontaneous liver cancer may be more suitable for studying various tumor microenvironments.[27] Therefore, we constructed a spontaneous HCC model by hydrodynamic tail vein injection of plasmids encoding myr‐AKT1and N‐RasV12 along with a sleeping beauty transposase to induce orthotopic HCC in mice.[28] Since the myr‐AKT1 plasmids carry a fragment of the luciferase gene, we were able to observe the size of the liver cancer in mice using the IVIS Spectrum system. This evidence concerns the gene AKT1 and neoplasm.