AKT1 and hepatocellular carcinoma: A transposon system containing plasmids encoding oncogenes along with transposase‐induced spontaneous liver cancer may be more suitable for studying various tumor microenvironments.[27] Therefore, we constructed a spontaneous HCC model by hydrodynamic tail vein injection of plasmids encoding myr‐AKT1and N‐RasV12 along with a sleeping beauty transposase to induce orthotopic HCC in mice.[28] Since the myr‐AKT1 plasmids carry a fragment of the luciferase gene, we were able to observe the size of the liver cancer in mice using the IVIS Spectrum system.