Herein, R54 reduced Foxp3 and DNMT1 transcription, as previously reported in murine ovarian cancer, where the CXCR4 inhibitor, AMD3100, selectively reduced intratumoral Foxp3 Tregs [23] and DNMT1 determining loss of Tregs suppressive function in vitro and in vivo [56, 60]. This evidence concerns the gene CXCR4 and ovarian carcinoma.