To enhance our comprehension of DMD and expedite its drug development, this study endeavoured to engineer microminipigs through the co-injection of embryos with the Cas9 protein and a single-guide RNA directed at exon 23 of DMD. This makes them highly suitable as models for studying human DMD and helps in predicting the potential effects of treatments and interventions in the disease with greater accuracy. This evidence concerns the gene DMD and Duchenne muscular dystrophy.