PUM3 and nasopharyngeal carcinoma: To reactivate the EBV lytic cycle in EBVaGC and NPC cells, we first exploited the efficient CRISPR-based Casilio activator system to induce the endogenous expression of the EBV-encoded immediate early lytic gene BZLF1. The Casilio activator system consists of a dCas9 protein, a PUFa-p65HSF1 activator module, and a sgRNA appended with five copies of PBSa12.