HBA2 and thalassemia: And for the αβ-thalassemia, RBC (Cut off≥4.77, PR=44.51%, DR=100.00%), RDW-CV (Cut off≥13.21, PR=22.55%, DR=83.33%), HbA2 (Cut off≥2.90, PR=17.78%, DR=95.83%), formula 5 (Cut off≥ 335.92, PR=46.38%, DR=83.33%), and formula 6 (Cut off≥ 609.79, PR=43.38%, DR= 83.33%) were suitable.