NPPB and Myocardial fibrosis: Furthermore, wheat germ agglutinin (WGA) staining, and immunoblotting of ANP, BNP and β-MHC indicated significant cardiomyocyte hypertrophy (Fig. 1i,j,l,m and Extended Data Fig. 2f,g), while Masson’s trichrome staining and collagen I content of the heart revealed aggravated myocardial fibrosis (Fig. 1i,k and Extended Data Figs. 1e and 2f,h) in TGR5ΔCM mice compared to TGR5fl/fl mice.